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Butternut squash is usually cut into 1/2" to 1" cubes, or sliced into rounds before being placed in the bag in a single layer. It is often tenderized when cooked for 45 to 60 minutes at 183°F to 185°F (83.9°C to 85°C), or a little longer if you puree it.
The length of time needed to sous vide butternut squash can vary depending on how ripe it is and the size of the pieces, but it is often tenderized when cooked for 45 to 60 minutes at 183°F to 185°F (83.9°C to 85°C). It is usually cut into 1/2" to 1" cubes, or sliced into rounds before being placed in the bag in a single layer. If your end dish will be pureed, then a longer cooking time will tenderize it even more tender.
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